Journal: Journal of Translational Medicine

Article Title: FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma

doi: 10.1186/s12967-021-03089-6

Figure Lengend Snippet: FAM83A overexpression in HN4 and HN6 cells promotes cell growth and metastasis. A , B FAM83A expressions based on real-time PCR and western blotting in HN4 and HN6 cell lines after treated with LV-FAM83A. C Morphological changes of HN4 and HN6 cells after LV-FAM83A transfection (100 ×). D CCK8 assays of the proliferation in HN4 and HN6 cells after LV-FAM83A transfection. E , F A wound-healing assay in HN4 and HN6 cells after LV-FAM83A transfection (100 ×). G , H Transwell assays of migration and invasion in HN4 and HN6 cells after LV-FAM83A transfection (100 ×). Data represent the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The human normal oral keratinocytes (HOK) and the human HNSCC cell lines including CAL27, FADU, HN4, HN6 SCC-9, and SCC-25 cell, were purchased from China Center for Type Culture Collection (Shanghai, China), CAL27 and HOK were cultured in DMEM medium (Gibco).

Techniques: Over Expression, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Wound Healing Assay, Migration

Journal: Journal of Translational Medicine

Article Title: FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma

doi: 10.1186/s12967-021-03089-6

Figure Lengend Snippet: FAM83A promotes EMT and activates Wnt/β-catenin signaling pathwayin HNSCC cells. A Protein levels of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail and Wnt-responsive genes including β-catenin, phosphor-β-catenin, c-myc and Cyclin D1 were determined by western blotting in CAL27 and FADU cells after shFAM83A transfection. B , C Gene expressions of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail, c-myc and Cyclin D1 were measured by RT-PCR in CAL27 and FAUD cells after shFAM83A transfection. D Protein levels of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail and Wnt-responsive gene including β-catenin, phosphor-β-catenin, c-myc and Cyclin D1 were determined by western blotting in HN4 and HN6 cells after LV-FAM83A transfection. E , F Gene expressions of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail and c-myc and Cyclin D1 were measured by RT-PCR in HN4 and HN6 cells after LV-FAM83A transfection. G Immunofluorescence analysis of the EMT marker E-cadherin and Vimentin in CAL27 cells after shFAM83A transfection (200 ×). H Immunofluorescence analysis of the EMT marker E-cadherin and Vimentin in HN6 cells after LV-FAM83A transfection (200 ×). Data represent the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The human normal oral keratinocytes (HOK) and the human HNSCC cell lines including CAL27, FADU, HN4, HN6 SCC-9, and SCC-25 cell, were purchased from China Center for Type Culture Collection (Shanghai, China), CAL27 and HOK were cultured in DMEM medium (Gibco).

Techniques: Western Blot, Transfection, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Marker

Journal: 3 Biotech

Article Title: Integrated strategy of temperature shift and mannitol feeding for enhanced production of echinocandin B by Aspergillus nidulans CCTCC M2012300

doi: 10.1007/s13205-019-1668-x

Figure Lengend Snippet: Comparison of different fermentation parameters at different temperature control conditions

Article Snippet: As shown in Fig. a, the dry cell weight (DCW) of A. nidulans CCTCC M2012300 increased quickly in the initial stage of fermentation and reached the maximum at the stationary phase for all operating temperatures.

Techniques: Comparison, Control

Journal: 3 Biotech

Article Title: Integrated strategy of temperature shift and mannitol feeding for enhanced production of echinocandin B by Aspergillus nidulans CCTCC M2012300

doi: 10.1007/s13205-019-1668-x

Figure Lengend Snippet: Effects of culture temperature on DCW (a), residual mannitol concentration (b), and ECB production (c) of A. nidulans CCTCC M2012300 in batch fermentation. The symbols used were 23 °C (unfilled diamond), 25 °C (filled triangle), 27 °C (unfilled circle) and 30 °C (filled inverted triangle)

Article Snippet: As shown in Fig. a, the dry cell weight (DCW) of A. nidulans CCTCC M2012300 increased quickly in the initial stage of fermentation and reached the maximum at the stationary phase for all operating temperatures.

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi: 10.1038/srep35131

Figure Lengend Snippet: Changes in the geometrical parameters of HCCs and NHs before and after CII.

Article Snippet: Five HCC lines, HepG2, Smmc-7721, Huh-7, Mhcc-97L, and Mhcc-97H, and a control NH line, L02, were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: Cell Analysis, Single-cell Analysis

Journal: Scientific Reports

Article Title: Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi: 10.1038/srep35131

Figure Lengend Snippet: ( A ) Cell shape vs mechanical properties. In detail, CPA vs E, in L02, r = 0.642, P = 0.00 < 0.01; in HepG2, r = 0.619, P = 0.00 < 0.01; in Smmc-7721, r = 0.519, P = 0.00 < 0.01; in Huh-7, r = 0.564, P = 0.00 < 0.01; in Mhcc-97L, r = 0.355, P = 0.005 < 0.01; ( B ) Cytoskeleton density vs mechanical properties. In detail, CD vs E, in L02, r = 0.872, P = 0.00 < 0.01; in HepG2, r = 0.790, P = 0.00 < 0.01; in Smmc-7721, r = 0.876, P = 0.00 < 0.01; in Huh-7, r = 0.866, P = 0.00 < 0.01; in Mhcc-97L, r = 0.724, P = 0.00 < 0.01; (C) Cell shape vs cytoskeleton density. In detail, CPA vs CD, in L02, r = 0.841, P = 0.00 < 0.01; in HepG2, r = 0.784, P = 0.00 < 0.01; in Smmc-7721, r = 0.700, P = 0.00 < 0.01; in Huh-7, r = 0.568, P = 0.00 < 0.01; in Mhcc-97L, r = 0.501, P = 0.003 < 0.01; ( D ) Caspase-3 protein levels vs mechanical properties. In detail, CPLs vs E, in L02, r = −0.811, P = 0.00 < 0.01; in HepG2, r = −0.784, P = 0.00 < 0.01; in Smmc-7721, r = −0.714, P = 0.00 < 0.01; in Huh-7, r = −0.747, P = 0.00 < 0.01; in Mhcc-97L, r = −0.701, P = 0.003 < 0.01.

Article Snippet: Five HCC lines, HepG2, Smmc-7721, Huh-7, Mhcc-97L, and Mhcc-97H, and a control NH line, L02, were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: